Look at this presentation by Airlie McCoy - it answers some of your questions. https://www.ccp4.ac.uk/schools/DLS-2014/course_material/day07/Airlie_McCoy_Phaser_MR.pdf
The crystal symmetry is taken into account when choosing the range of angles to search. If you run MOLREP - another more limited molecular replacement program available from CCP4 - it does give a listing of all symmetry equivalents of the rotation function peaks which is sometimes useful.. On Sat, 25 Jan 2025 at 09:48, zx2...@connect.hku.hk <zx2...@connect.hku.hk> wrote: > Dear All, > I hope this email finds you well. I would like to clarify how the Phaser > program determines the search range and step size for both the rotation and > translation searches. Specifically, I am wondering: > > 1. *Rotation search range*: What are the typical angular ranges for α, > β, and γ? For instance, is it always 0–360° for α and γ, and 0–180° for β? > 2. *Step size*: How is the angular step determined? Are α, β, and > γ sampled at the same increments, or does Phaser adjust them independently? > 3. *Crystal symmetry*: Does the symmetry of the crystal affect the > search range or step size? If so, in what manner does it reduce or modify > the search space? > 4. *Documentation*: Where can I find detailed references or > documentation that discusses these parameters? > > I would greatly appreciate any advice, references, or practical tips on > how to handle these settings properly. Thank you in advance for your help! > Best regards, > Xin > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
