Dear Paul, Thank you
Regards Devbrat On Thu, Jun 6, 2024, 8:53 AM Paul Emsley <pems...@mrc-lmb.cam.ac.uk> wrote: > > On 06/06/2024 04:00, Devbrat Kumar wrote: > > > -- > Dear Paul, > > Thank you for your response. I wanted to compare a Coulomb potential map > to an electron density map. Before aligning these maps, I need to bring > them to similar parameters, which requires rescaling one map to match the > other. After that, I can proceed with density subtraction. > > I hope this clarifies my query. Sorry for any confusion. > Thank you again. > Regards > Devbrat > > > On Wed, Jun 5, 2024, 7:56 PM Paul Emsley <pems...@mrc-lmb.cam.ac.uk> > wrote: > >> >> On 05/06/2024 07:00, Devbrat Kumar wrote: >> >> >> -- >> >> Hello Everyone, >> >> Hello Devbrat, >> >> I have a query regarding the resampling of cryoEM density to match >> crystal density to obtain a density difference map. Specifically, I am >> trying to determine if it is feasible to resample a cryoEM map with an XRD >> density map. However, each time I attempt this, the resampling output >> provides an arbitrary ASU resample map, resulting in a significant loss of >> major density. >> >> I have been using Coot and Chimera for this process but have not achieved >> the desired outcome. Please guide me or suggest how to move forward with >> this. My goal is to create an accurate final density difference map. >> >> >> It is not clear to me exactly what the problem is. >> >> In Coot speak, "resampling" is (merely) changing the grid sampling so >> that the map appears (typically) on a finer grid. >> >> I don't think that this is what you want. >> >> You want is, I think, "Transform Map by LSQ Model-fit" and if that is >> what you used, then I can't help until I am more clear about what you think >> has gone wrong. >> >> Regards, >> >> Paul. >> >> Ah, OK, so you want to *rescale* not resample. The tool (in our world) to > do that is EMDA (available in CCPEM). > > Paul. > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
