On 05/06/2024 07:00, Devbrat Kumar wrote:
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Hello Everyone,
Hello Devbrat,
I have a query regarding the resampling of cryoEM density to match
crystal density to obtain a density difference map. Specifically, I am
trying to determine if it is feasible to resample a cryoEM map with an
XRD density map. However, each time I attempt this, the resampling
output provides an arbitrary ASU resample map, resulting in a
significant loss of major density.
I have been using Coot and Chimera for this process but have not
achieved the desired outcome. Please guide me or suggest how to move
forward with this. My goal is to create an accurate final density
difference map.
It is not clear to me exactly what the problem is.
In Coot speak, "resampling" is (merely) changing the grid sampling so
that the map appears (typically) on a finer grid.
I don't think that this is what you want.
You want is, I think, "Transform Map by LSQ Model-fit" and if that is
what you used, then I can't help until I am more clear about what you
think has gone wrong.
Regards,
Paul.
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