Also forget to observe side chains at 4A! At this resolution is crucial
to critically assess the position of each amino acid in your model,
taking into account factors such as hinges in your structure,
hydrophobic and hydrophilic regions, and the types of contacts between
secondary structure elements. Paying attention to these details can
enhance the accuracy and reliability of your model.
Good luck!
Albert Castellví
On 11/21/23 20:57, Albert Castellvi Toledo wrote:
Hello,
At low resolutions, model bias becomes a significant issue, capable of
yielding inaccurate MR solutions even with favorable figures of merit.
In my experience, it is always necessary to validate MR solutions at
low resolution. One effective approach involves systematically
omitting small fragments from the model and see if the resulting maps
suggest you the placement of the omitted fragment or not. This method
enables the identification of whether the solution is genuinely robust
or if it is highly influenced by model bias.
Salut,
Albert Castellví
On 11/21/23 13:02, Yahui Liu wrote:
Dear all,
I got a protein crystal dataset of 4.3 A and would like to some the
structure with MR.
Now I am suffering with the refinement. I used both Refmac and Phenix.
Someone could give me a hand or any suggestions?
All the best
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