Hello,
At low resolutions, model bias becomes a significant issue, capable of
yielding inaccurate MR solutions even with favorable figures of merit.
In my experience, it is always necessary to validate MR solutions at low
resolution. One effective approach involves systematically omitting
small fragments from the model and see if the resulting maps suggest you
the placement of the omitted fragment or not. This method enables the
identification of whether the solution is genuinely robust or if it is
highly influenced by model bias.
Salut,
Albert Castellví
On 11/21/23 13:02, Yahui Liu wrote:
Dear all,
I got a protein crystal dataset of 4.3 A and would like to some the
structure with MR.
Now I am suffering with the refinement. I used both Refmac and Phenix.
Someone could give me a hand or any suggestions?
All the best
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