Dear All If anyone has a DNA for an e.coli vector for production of
human rhinovirus (HRV) 3C protease for cleaving off fusion partners/tags that they would be happy to share that would be amazing. GST-tagged or otherwise. Single step purification would be great M.G. Cordingley, P.L. Callahan, V.V. Sardana, V.M. Garsky, R.J. Colonno, Substrate requirements of human rhinovirus 3C protease for peptide cleavage in vitro, J. Biol. Chem. 265 (1990) 9062–9065. Yours jonas ################################## Dr Jonas Emsley Professor of Macromolecular Crystallography, Nottingham Biodiscovery Institute School of Pharmacy, University of Nottingham, University Park, Nottingham. NG72RD Tel: +44 1158467092 Fax: +44 1158468002 email:jonas.ems...@nottingham.ac.uk<mailto:jonas.ems...@nottingham.ac.uk> http://www.nottingham.ac.uk/research/groups/structural-biology/index.aspx [signature_3865098345]https://orcid.org/0000-0002-8949-8030 This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please contact the sender and delete the email and attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. Email communications with the University of Nottingham may be monitored where permitted by law. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
