On 14/07/2023 08:52, Jonas Emsley wrote:
Dear All
We have a crystal structure with O-glycans for the first time attached
to a Ser and a Thr sidechain
If anyone can recommend the procedure for adding these in coot and
refining them in phenix /refmac that would be greatly appreciated
Hi Jonas,
I didn't get around to adjusting Coot's carbohydrate to build O-linked
glycans :-(.
If I wanted to build one today, I'd have to use cut, paste, superpose,
copy fragment, merge molecules and change chain-ids. Meh.
Once you have built it however, and used Make Link to add the link to
the header, the refinement linking should be automatic in Coot (because
the details of the link are in the CCP4 monomer library).
Regards,
Paul.
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