Hi Jürgen You might think so, but I’d disagree. Not going too far away from your line of reasoning I could also put in a completely fictitious ligand or cofactor and assign its occupancies to zero (I really, really knew it was there but I just couldn’t find any evidence… :-))
Harry > On 10 Mar 2023, at 16:58, Jurgen Bosch <jxb...@case.edu> wrote: > > Going back to RIP phasing methods :-) > So Harry in your particular case occupancy of zero would actually reflect > reality for those “combusted” atoms. > > Jürgen > >> On Mar 10, 2023, at 11:56 AM, Harry Powell >> <0000193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote: >> >> Hi folks >> >> One other thing that I haven’t noticed anyone mentioning yet (sorry to those >> who have mentioned it!!) is that you may not see your sidechain atoms in >> density because they are not there at all, in spite of what you may have had >> in the original protein, or even if the atoms were really there in the >> crystal _before_ exposure to the beam. >> >> The coordinates are supposed to be what you actually find, not what you hope >> is there. >> >> Just my two ha’porth >> >> Harry >> >> ######################################################################## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> >> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing >> list hosted by www.jiscmail.ac.uk, terms & conditions are available at >> https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
