Dear Gyuhyeok, I have no first-hand experience with CARELESS.
But my understanding is that CARELESS gives you normalized structure factor amplitudes; this "normalization" roughly corresponds to a removal (subtraction) of the Wilson B-factor from conventionally processed structure factor amplitudes. As a result, you'd expect maps that appear sharpened relative to what we normally look at, and a Wilson B-factor around 0. So this is all consistent with what you report. It is unclear to me whether one can or should refine against data from CARELESS without taking extra measures, and whether and how the data can be deposited with the PDB. At least they should be suitably flagged. HTH, Kay ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
