Hi all, This is my first JISC post so I am still working on how to navigate this. I have to say I feel like there is a much better way to have a forum on structural biology... I could imagine a discord server where we can post individual questions, have live chats, etc. I'd be happy to set this up if people are interested.
The data collection was done on a single crystal, using vector scanning to minimize radiation damage, and using oil to prevent dehydration. I have since gone back and re-processed the 313K dataset with an updated DIALS and the problem seems to have gone away (and gave me better scaling statistics)...my guess is that the scaling program is just improving some of these "bugs". This high temp dataset now has the highest average B-factor. Regardless, there does seem to be not much variation with the temperature at the modest temperatures which may be in line with the biological story but these values make much more sense now. Thanks everyone for all the suggestions with regards to scaling the data together, SCALEIT, etc. I have a few different options to interpret the data now. Matt ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
