Murpholino, You may want to consider where your protein is in relation to the Unit Cell and the Asymmetric Unit. Use the COOT File/Save Symmetry Coordinates/ to choose the symmetry molecule that fits the CCP4 map.
Best regards, Mark Mark Andrew White, Ph.D. Associate Professor of Biochemistry & Molecular Biology, Manager, SCSB Macromolecular X-ray Laboratory 6.658A Basic Sciences L#35115, UTMB, Galveston, TX 409.747.4747 http://xray.utmb.edu ________________________________ From: Mailing list for users of COOT Crystallographic Software <c...@jiscmail.ac.uk> on behalf of Murpholino Peligro <murpholi...@gmail.com> Sent: Friday, July 23, 2021 10:26 AM To: c...@jiscmail.ac.uk <c...@jiscmail.ac.uk> Subject: Re: Move fem map back to protein? WARNING: This email originated from outside of UTMB's email system. Do not click links or open attachments unless you recognize the sender and know the content is safe. If I open the fem.mtz file and choose FEM and PHIFEM for amplitudes and phases I get a map (cyan) over my protein. If I open the fem.ccp4 file the map (yellow) is not over my protein. I guess I will work with the mtz file. El vie, 23 de jul. de 2021 a la(s) 09:50, Murpholino Peligro (murpholi...@gmail.com<mailto:murpholi...@gmail.com>) escribió: Dear all.. How do I move a fem map back to the protein molecule? I did `phenix.fem PHASER.1-coot-1_refine_001.pdb PHASER.1-coot-0_refine_data.mtz` to generate the fem map, but when I open with coot `coot PHASER.1-coot-1_refine_001.mtz PHASER.1-coot-1_refine_001.pdb` and then file->open map->fem.cpp4... the fem map does not cover the protein (as shown in .png attached) I am not sure if this is phenix (a missing option?) or coot related ... thanks ________________________________ To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=COOT&A=1<https://nam11.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCOOT%26A%3D1&data=04%7C01%7Cmawhite%40UTMB.EDU%7Cd62cd10d817247432bec08d94dee7ae0%7C7bef256d85db4526a72d31aea2546852%7C0%7C1%7C637626508942966734%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=bGiEbDupGqi7sffMSmtE1jTRChGfOOSSVC8p9K3XmTE%3D&reserved=0> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
