Hello, yes, the difference map looks great. If this one diffracts to near-atomic resolution, maybe you have a tetramer with a very low solvent content. Cheers, Jon.C..
Sent from ProtonMail mobile -------- Original Message -------- On 5 Jul 2021, 14:54, Robert S Phillips wrote: > I collected data last week on crystals of tyrosine phenol-lyase obtained > under new conditions. The data have higher resolution than previous crystals, > to 1.5 A. However, I can't get them to index in any space group but P1. > Usually, the space group is P21212. The self-rotation function is attached. > The P1 data will give a molecular replacement solution, but it does not > refine below 0.46. The P1 asymmetric unit fits a dimer, but the assembly is a > tetramer. In the map, I can see the difference peaks from the other dimer of > the tetramer. What could be causing this problem? > > Rob > > Robert S. Phillips > Professor of Chemistry and of Biochemistry and Molecular Biology > University of Georgia > Athens, GA 30602 > Phone: (706) 542-1996 > Fax: (706) 542-9454 > E-mail: rsphill...@chem.uga.edu > Web: > [http://tryptophan.net](https://pod51004.outlook.com/owa/redir.aspx?C=ccbf42ffea5f48b1bf8e9bb950454bab&URL=http%3a%2f%2ftryptophan.net) > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
