Why you have this problem I cannot guess, but your self-rotation clearly
shows 2 2 2 symmetry.
Try another data processing system?
Eleanor


On Mon, 5 Jul 2021 at 14:54, Robert S Phillips <p...@uga.edu> wrote:

> I collected data last week on crystals of tyrosine phenol-lyase obtained
> under new conditions.  The data have higher resolution than previous
> crystals, to 1.5 A.  However, I can't get them to index in any space group
> but P1.  Usually, the space group is P21212.  The self-rotation function is
> attached.  The P1 data will give a molecular replacement solution, but it
> does not refine below 0.46.  The P1 asymmetric unit fits a dimer, but the
> assembly is a tetramer.  In the map, I can see the difference peaks from
> the other dimer of the tetramer.  What could be causing this problem?
>
> Rob
>
> Robert S. Phillips
> Professor of Chemistry and of Biochemistry and Molecular Biology
> University of Georgia
> Athens, GA 30602
> Phone: (706) 542-1996
> Fax: (706) 542-9454
> E-mail: rsphill...@chem.uga.edu
> Web:  http://tryptophan.net
> <https://pod51004.outlook.com/owa/redir.aspx?C=ccbf42ffea5f48b1bf8e9bb950454bab&URL=http%3a%2f%2ftryptophan.net>
>
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