We developed a senior undergraduate biochemistry lab around an acid phosphatase from Francisella tularensis (FtHAP).
https://pubmed.ncbi.nlm.nih.gov/27980518/ The enzyme is easy to purify and crystallize, and the crystals diffract well. L-tartrate and phosphate ion are inexpensive inhibitors, which can be included in crystallization (3IT0/1). We have the students grow crystals and collect X-ray diffraction data remotely. Then they view maps in Coot. The enzyme assay is a simple colorimetric test with p-nitrophenylphosphate as the substrate. -- John J. Tanner Professor of Biochemistry and Chemistry Associate Chair of Biochemistry Department of Biochemistry University of Missouri 117 Schweitzer Hall 503 S College Avenue Columbia, MO 65211 Phone: 573-884-1280 Email: tanne...@missouri.edu<mailto:tanne...@missouri.edu> https://cafnrfaculty.missouri.edu/tannerlab/ Lab: Schlundt Annex rooms 3,6,9, 203B, 203C Office: Schlundt Annex 203A From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of P. H <duskyde...@gmail.com> Date: Wednesday, June 16, 2021 at 5:19 PM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Looking for proteins for undergraduate biochemistry lab WARNING: This message has originated from an External Source. This may be a phishing expedition that can result in unauthorized access to our IT System. Please use proper judgment and caution when opening attachments, clicking links, or responding to this email. Hello All, We are looking for some candidate proteins for an undergraduate level advanced biochemistry lab. They should be expressed in bacteria, simple enough to purify and it will be nice to perform some simple characterization experiments(binding assays, enzymatic assays). Any suggestions? Thank you in advance. Prerna gupta ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=04%7C01%7C%7Cd8b39471bfe34f36491308d93114d507%7Ce3fefdbef7e9401ba51a355e01b05a89%7C0%7C0%7C637594787813433934%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=Lqfa7oQfLmca246Xyr4FzEUz1srrP53XVqWSh%2B9vSng%3D&reserved=0> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
