Hi Peer, Reminds me of a lipocalin structure I solved which co-purified fatty acid with it. I could model fatty acids and we along with our collaborators used several complementary biophysical methods followed by functional assays to identify the ligand. https://www.sciencedirect.com/science/article/abs/pii/S002228360901078X [https://ars.els-cdn.com/content/image/1-s2.0-S0022283609X00422-cov150h.gif]<https://www.sciencedirect.com/science/article/abs/pii/S002228360901078X> Serendipitous Fatty Acid Binding Reveals the Structural Determinants for Ligand Recognition in Apolipoprotein M<https://www.sciencedirect.com/science/article/abs/pii/S002228360901078X> Apolipoprotein M (ApoM) is a 25-kDa HDL-associated apolipoprotein and a member of the lipocalin family of proteins. Mature apoM retains its signal pep… www.sciencedirect.com Thank you. All the best, Madhu
---------------------------------------------------- Madhumati Sevvana, Ph.D. Assistant Research Scientist Structural Virology lab of Prof. Richard Kuhn Hockmeyer Hall of Structural Biology Department of Biological Sciences Purdue University, West Lafayette IN ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Peer Mittl <mi...@bioc.uzh.ch> Sent: Thursday, June 3, 2021 06:50 To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] unknown density in 1.6 A structure I'm struggling with the electron density shown in the attachment. The substance does not seem to come from the crystallization condition nor from the mounting buffer. The 1.6 A maps are rather clear, because the compound binds into the hydrophobic core, which seems to be loosely packed. -Peer ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB<http://www.jiscmail.ac.uk/CCP4BB>, a mailing list hosted by www.jiscmail.ac.uk<http://www.jiscmail.ac.uk>, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
