Hi Peer,
this density reminds me of something I saw in one of my own structures as well and which also was not a component that should have been in there (PDB ID 6SMT). I never truly found out what it was, but I modeled it as 2-ethylhexanol, which is known to be a precursor for the synthesis and also degradation product of the wide-spread plasticizer DEHP, although I found out, that at least our crystallization plates do not contain this component. Best, Timo ________________________________ Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> im Auftrag von Peer Mittl <mi...@bioc.uzh.ch> Gesendet: Donnerstag, 3. Juni 2021 12:50:00 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] unknown density in 1.6 A structure I'm struggling with the electron density shown in the attachment. The substance does not seem to come from the crystallization condition nor from the mounting buffer. The 1.6 A maps are rather clear, because the compound binds into the hydrophobic core, which seems to be loosely packed. -Peer ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB<http://www.jiscmail.ac.uk/CCP4BB>, a mailing list hosted by www.jiscmail.ac.uk<http://www.jiscmail.ac.uk>, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
