Finding a solution will be very difficult then. Eleanor On Fri, 23 Apr 2021 at 21:11, Swati Gupta <visvasw...@gmail.com> wrote:
> My data is moderate anisotropic also with a high Wilson b factor greater > than 200 > > On Sat, 24 Apr, 2021, 01:19 Eleanor Dodson, <eleanor.dod...@york.ac.uk> > wrote: > >> If you were lucky the new crystal might have the same cell and >> spacegroup as your model, but otherwiseThis is a case for molecular >> replacement.? >> >> My course of action using CCP4I2 >> >> Process data carefully and look for any warnings. >> Align your new sequence with the model using clustalw >> Edit the model to have the new sequence - SCULTOR does this >> If the cells are the same you can just start refinement against the new >> data with the model in the same position . >> But if not >> Run PHASER or MOLREP to find the new orientation for the model. >> >> Now is the really difficult part - refining and rebuilding a structure >> with low resolution data. >> Maybe best to look for a better crystal! >> Eleanor. >> >> On Fri, 23 Apr 2021 at 20:26, Swati Gupta <visvasw...@gmail.com> wrote: >> >>> Dear all, >>> how do I proceed with solving a protein structure with >>> 3.9 A resolution and an i/sig i of 1.3 and cchalf of 0.8. The homologous >>> protein has 48% identity to be used as template. Kindly help with what >>> programs to run. >>> >>> >>> Thanks >>> Swati >>> >>> ------------------------------ >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >>> >> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
