My data is moderate anisotropic also with a high Wilson b factor greater than 200
On Sat, 24 Apr, 2021, 01:19 Eleanor Dodson, <eleanor.dod...@york.ac.uk> wrote: > If you were lucky the new crystal might have the same cell and > spacegroup as your model, but otherwiseThis is a case for molecular > replacement.? > > My course of action using CCP4I2 > > Process data carefully and look for any warnings. > Align your new sequence with the model using clustalw > Edit the model to have the new sequence - SCULTOR does this > If the cells are the same you can just start refinement against the new > data with the model in the same position . > But if not > Run PHASER or MOLREP to find the new orientation for the model. > > Now is the really difficult part - refining and rebuilding a structure > with low resolution data. > Maybe best to look for a better crystal! > Eleanor. > > On Fri, 23 Apr 2021 at 20:26, Swati Gupta <visvasw...@gmail.com> wrote: > >> Dear all, >> how do I proceed with solving a protein structure with >> 3.9 A resolution and an i/sig i of 1.3 and cchalf of 0.8. The homologous >> protein has 48% identity to be used as template. Kindly help with what >> programs to run. >> >> >> Thanks >> Swati >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
