Hi, Looks like, you have the solution. Have you tried the Phenix AutoBuild module? sometimes it works well.
Best luck On Tue, Feb 16, 2021 at 1:21 PM Muhammad Bashir Khan <m...@ualberta.ca> wrote: > Dear All; > > I have data set of about 3.0A. It's a multidomain protein. I tried several > options with MR but it's was not working. I trimmed one of the > search models, it does not give any solution using CCP4 molrep. > > I used Phenix phaser it gives a solution after several hours with the > following output. > > ** SINGLE solution > > ** Solution written to PDB file: MgtA_phaser.1.pdb > ** Solution written to MTZ file: MgtA_phaser.1.mtz > Solution annotation (history): > SOLU SET RFZ=2.7 TFZ=6.3 PAK=6 LLG=62 TFZ==6.5 RFZ=2.2 TFZ=28.0 PAK=9 > LLG=1207 TFZ==42.0 LLG=1370 TFZ==41.8 PAK=9 > LLG=1370 TFZ==41.8 > SOLU SPAC P 21 2 21 > SOLU 6DIM ENSE ense_1 EULER 269.0 80.4 177.3 FRAC 0.24 -0.22 -0.07 > BFAC -1.51 #TFZ==6.5 > SOLU 6DIM ENSE ense_1 EULER 89.0 80.4 177.3 FRAC 0.26 -1.01 -0.07 > BFAC 2.26 #TFZ==41.8 > SOLU ENSEMBLE ense_1 VRMS DELTA -0.7291 #RMSD 1.50 #VRMS 1.23 > > Running Phenix refine on the solution gives a breakup map and the R > factors are not decreasing below 47.61 and 50.94. > > Thank you for any suggestions in advance > > Regards; > > Bashir > > > -- > ------------------------------------------------------ > Muhammad Bashir Khan, Ph.D. > Research Associate > Department of Biochemistry > Medical Science Bldg. > Lab 3-27 > University of Alberta > Edmonton AB, T6G 2H7 > > Phone: 780-492-4577- > e-mail: m...@ualberta.ca <cbroo...@ualberta.ca> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > -- Sharan Karade Postdoc-fellow IBBR-UMD, 9600 Gudelsky Dr, Rockville Maryland 20850 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
