Dear All;
I have data set of about 3.0A. It's a multidomain protein. I tried several
options with MR but it's was not working. I trimmed one of the
search models, it does not give any solution using CCP4 molrep.
I used Phenix phaser it gives a solution after several hours with the
following output.
** SINGLE solution
** Solution written to PDB file: MgtA_phaser.1.pdb
** Solution written to MTZ file: MgtA_phaser.1.mtz
Solution annotation (history):
SOLU SET RFZ=2.7 TFZ=6.3 PAK=6 LLG=62 TFZ==6.5 RFZ=2.2 TFZ=28.0 PAK=9
LLG=1207 TFZ==42.0 LLG=1370 TFZ==41.8 PAK=9
LLG=1370 TFZ==41.8
SOLU SPAC P 21 2 21
SOLU 6DIM ENSE ense_1 EULER 269.0 80.4 177.3 FRAC 0.24 -0.22 -0.07
BFAC -1.51 #TFZ==6.5
SOLU 6DIM ENSE ense_1 EULER 89.0 80.4 177.3 FRAC 0.26 -1.01 -0.07
BFAC 2.26 #TFZ==41.8
SOLU ENSEMBLE ense_1 VRMS DELTA -0.7291 #RMSD 1.50 #VRMS 1.23
Running Phenix refine on the solution gives a breakup map and the R factors
are not decreasing below 47.61 and 50.94.
Thank you for any suggestions in advance
Regards;
Bashir
--
------------------------------------------------------
Muhammad Bashir Khan, Ph.D.
Research Associate
Department of Biochemistry
Medical Science Bldg.
Lab 3-27
University of Alberta
Edmonton AB, T6G 2H7
Phone: 780-492-4577-
e-mail: m...@ualberta.ca <cbroo...@ualberta.ca>
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