Dear All,
We have recently performed a thermal melt curve analysis using
DSF for a target protein using SYPRO-Orange Dye.
We are extending our studies on the same protein with time-lapse experiment
(ie., RFU Vs Time) at a constant temperature (say 37 degC) using the same
extrinsic SYPRO-Orange Dye. While RFU's are positive for melt curves, We
see RFU's in -ve range for the time-lapse curve. We are using the
instrument *RT-PCR (Biorad CFX96).*
We would be grateful for any suggestions on related literature
or protocol for the time-lapse experimental strategy.
Many thanks for your help.
Best regards,
Aadi
Aadithye R Nair
Dr. R. Natesh NSMB Lab (Natesh Structural Molecular Biology Lab)
Indian Institute of Science Education and Research Thiruvananthapuram
(IISER-TVM)
Thiruvananthapuram 695551,
Kerala, INDIA
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