Hi Joseph Great diffraction patterns. As Jon has just said, it could be zinc acetate. There are some very strong spots which would be consistent with a single crystal of this. However, something else seems to be present. Could it instead be PEG crystals with some superlattice repeat? For unit cell dimensions of PEG see https://pubs.acs.org/doi/abs/10.1021/ma60035a005 Some circles giving Bragg spacings would help. Also the rotation range. You should at least be able to estimate some of the cell dimensions and see if they are consistent with PEG, zinc acetate or some alternative. I seem to recall the subject of PEG crystals coming up before. Colin
-----Original Message----- From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Joseph Ho Sent: 08 December 2020 15:47 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] seek your opion on this weird diffractio pattern Dear all: We recently worked on 8kDa protein crystal structure. We obtained crystals in Zinc acetate and PEG8000 condition. However, we observed this unusual diffraction patterns. I am wondering if anyone observed this and know how this can occur. The cryoprotectant is glycerol. Thank you for you help Joseph ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
