Hello, the first of those diffraction patterns is very beautiful. What was the concentration of zinc acetate? Also, did you keep the crystal? It might be worth prodding it with a needle and if holds together well or if you hear a loud crack it's probably salt. The second image shows a salt-like pattern. I think those lunes of spots in the first image are some sort of disorder in the crystal, which I fear is zinc acetate. Best wishes, Jon Cooper.
Sent from ProtonMail mobile -------- Original Message -------- On 8 Dec 2020, 15:46, Joseph Ho wrote: > Dear all: > We recently worked on 8kDa protein crystal structure. We obtained > crystals in Zinc acetate and PEG8000 condition. However, we observed > this unusual diffraction patterns. I am wondering if anyone observed > this and know how this can occur. The cryoprotectant is glycerol. > > Thank you for you help > > Joseph > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
