We no longer use the reusable bases for the same reasons, but we do re-use our bases. If you use superglue to glue the pins into the bases, you can remove the pins from the bases by soaking them in acetone. This works about 75% of the time, especially if you are careful not to use too much superglue when you glue the pin into the base.
It goes without saying that the use of acetone will dissolve the loop, but then the main reason we remove the pin is due to imperfections in the loop, so no big deal. Whenever we see the motions that you describe, we postulate that somehow the pins were damaged and they are loose in the base. Occasionally this motion is ascribed to ice between the base of the pin and the magnetic mount, but this happens very rarely in our experience. Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry UT Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu (214) 645-6383 (phone) (214) 645-6353 (fax) ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Tao-Hsin Chang <taohsin.ch...@gmail.com> Sent: Sunday, November 15, 2020 3:07 PM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Stabilizing Mitegen reusable bases/mounts EXTERNAL MAIL Hi Patrick, I have met the same issue and that is very bad for any micro-focus beam and small crystals. I have talked with Mitegen and they are developing a new type of reusable base having an improvement for this issue. You may get some update from them. But, I do not use the reusable bases anymore. Best wishes, Tao-Hsin On Nov 15, 2020, at 3:45 PM, Patrick Loll <pjl...@gmail.com<mailto:pjl...@gmail.com>> wrote: Hi everyone, I’ve become very fond of the Mitegen reusable bases for mounting crystals, since the reusable aspect savse me from having to discard the base every time I break a microloop. However, once the crystals arrive at the synchroteon, I observe motions of the loops (some gradual, some sporadic). The the amplitudes of these motions are becoming significant as I take data from smaller and smaller crystals. I don’t think I’m imagining this, since the good folks at NSLS-2/AMX have warned me about this very issue. I’m writing to ask if anyone has any clever ideas about stabilizing these assemblies. Obviously, I can epoxy the pins in place, but then I’ll probably need to discard the entire assembly when I break a loop, and I’d prefer not to waste more money than necessary. I’ve considered putting a bead of wax at the point where the pin enters the base (although I haven’t yet checked to see if that will survive immersion in liquid nitrogen). Does anyone have any other (better) ideas? Much obliged in advance, Pat __________________________ Patrick J. Loll, PhD Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St. Philadelphia, PA 19102-1192 USA (215) 762-7706 pj...@drexel.edu<mailto:pj...@drexel.edu> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 CAUTION: This email originated from outside UTSW. Please be cautious of links or attachments, and validate the sender's email address before replying. ________________________________ UT Southwestern Medical Center The future of medicine, today. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
