P53 tetramerization domain might be a way to go.

________________________________
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Comolettis 
<85822939...@gmail.com>
Sent: Friday, September 25, 2020 9:59 PM
To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] tetrameric tag for extracellular proteins

Hi all,

Following what someone recently suggested, Fc tag is a great way to dimerize a 
secreted protein. I have successfully tried the TNF receptor-associated factor 
1 tail to trimerize and the COMP (cartilage oligomeric matrix protein) to 
pentamerize my monomer but I can't identify a suitable tag to tetramerize my 
construct.
Any pointer?

Thanks,
Davide Comoletti
VUW, Wellington, NZ

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