Hi Yadav, yes, this is only a representation of what I would like to do. If during the refinement will continue to appear density, probably I'm on the good track......but currently my big issue is inserting the ACN covalently linked to both cys.
many thanks Daniele Veggi GSK Vaccines Siena Italy Il giorno mar 30 giu 2020 alle ore 12:52 Lumbini Yadav <lumbin...@gmail.com> ha scritto: > Hi Daniele, > Do you see Fo-Fc density near disuphide bond where you are trying to fit > acetone? > > On Tue, Jun 30, 2020 at 3:28 PM Daniele Veggi <veggidani...@gmail.com> > wrote: > >> Dear CCP4bb, >> >> I'm trying to insert an Acetone molecule between two cysteine residues in >> coot or modifying the pdb. I'm working on this modified molecule where the >> disulfide bridge was chemichally opened and inserted an acetone molecule >> between the two cysteine. >> >> MS data confirm this modification (98% of molecules modified) but I can >> not insert the acetone covalently linked to the S of both cysteine >> (S-ACN-S). >> Maybe what I think and what I am doing is not the right solution (the >> disulfide bridge is still alive?) anyway i would like to know how to link >> the acetone to the two cysteine >> To better understand what I mean in attach you find a screenshot >> >> I thank in advance all those who will have the courtesy to make a >> suggestion >> >> Daniele Veggi >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
