Hi Silvia, Looking at the end of the log file, it looks like you have a very clear solution and are ready to go!
This message means that a better solution from the Fast Translation Function was excluded because it failed the packing test (too many clashes with neighboring molecules). Sometimes this will prevent you from getting a usable solution altogether, and this message would clue you into that. In such a situation, you could prune flexible/non-conserved loops from your protein and try MR again with the truncated model. But in this case, your still managed to get a very clear solution, so I think you are ready to look at your maps and begin rebuilding and refinement. Good luck! Best, Damian > On Jun 16, 2020, at 4:47 PM, Napolitano Silvia > <silvia.napolit...@mol.biol.ethz.ch> wrote: > > > Dear ccp44 Bulletin Board, > > I am trying to solve a structure by molecular replacement. > I am doing that by using PhaserMR (simple one-component interface). > > I tried out setting up different parameters (e.g.changing # of components, > #search, different space group search settings...), no matter what I try I > always get this warning message: > > ------------------------------------------------------------------------------------------ > Advisory: The top solution from a FTF did not pack > ------------------------------------------------------------------------------------------ > > ------------------------------------------------------------------------------------------ > Advisory: The top solution from a TF rescoring did not pack > > (please, see log file attached). > > What does this Warning mean? I tried to look it up online but, unfortunately, > I could not find the answer I was looking for. > > Please, let me know if you need more detailed information. > > Many thanks in advance for your help and your time! > > Best Regards > > Silvia > > Silvia Napolitano > ETH Zurich > Institute of Molecular Biology and Biophysics > Otto-Stern-Weg 5, HPK E14 > CH–8093 Zurich > Phone: +41 44 6332482 > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <MsrAC198-C206_phaser.log> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
