Hi Jiyuan, I don't have much to add on the small molecule crystallization advice, but I will put in another plug for electron diffraction (microED). You could first check to see if you already have microcrystal by performing some X-ray powder diffraction or XRPD (many CROs offer this service). If your spectra looks good, you can move straight to data collection with electron diffraction. If you don't already have crystals, I would proceed as suggested with crystallization trials. If you get big crystals, great! Go for single crystal X-ray diffraction. If you find that you can only make microcrystals, I would try to do microED.
Best of luck! On Mon, Jun 1, 2020 at 3:01 PM Jiyuan Ke <jiyuan...@h3biomedicine.com> wrote: > Hi Everyone, > > I want to crystallize a small organic molecule. I have very limited > experience in small molecule crystallography. I found that the Crystal > Screen HT from the Hampton research is good for both small molecule and > macromolecule crystallization. Plan to set up a sitting drop screen just > like setting up protein crystallization. I don’t know if this is the proper > way to do it. Is the MRC sitting drop 2-well plate (HR3-083) used for > protein crystallization good for small molecule crystallization? Are there > any special plates used for small molecule crystallization? Is room > temperature ok or not? > > For data collection, can I use the beamline for protein crystals to > collect data on small molecule crystals? Larger oscillation angle, shorter > exposure, reduced beam intensity? > > For structure determination, is SHELXL the preferred software for solving > small molecule structures? > > If anyone has experience in small molecule crystallography, please help. > Thanks! > > Best Regards, > > -- > > *Jiyuan Ke, Ph.D.* > > > Research Investigator > > H3 Biomedicine Inc. > > 300 Technology Square, Floor 5 > > Cambridge, MA 02139 > > Phone: 617-252-3923 > > Email: jiyuan...@h3biomedicine.com > > Website: www.h3biomedicine.com > > > > > > > [This e-mail message may contain privileged, confidential and/or > proprietary information of H3 Biomedicine. If you believe that it has been > sent to you in error, please contact the sender immediately and delete the > message including any attachments, without copying, using, or distributing > any of the information contained therein. This e-mail message should not be > interpreted to include a digital or electronic signature that can be used > to authenticate an agreement, contract or other legal document, nor to > reflect an intention to be bound to any legally-binding agreement or > contract.] > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- Jessica Bruhn, Ph.D Principal Scientist NanoImaging Services, Inc. 4940 Carroll Canyon Road, Suite 115 San Diego, CA 92121 Phone #: (888) 675-8261 www.nanoimagingservices.com ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
