I take it that you have 2 molecules in the asymmetric unit. If so, you could try some sort of NCS averaging of the map or just NCS-refinement might help. Not much more here than has been expertly suggested already. Can you give us an idea of the resolution? How many RMS have you contoured the map at?
Jon Cooper (pre-polder person)
On 20 Apr 2020 20:23, Kyle Gregory <00003632e92fcc15-dmarc-requ...@jiscmail.ac.uk> wrote:
Hello all,
I have a homodimer structure in P1 21 1 spacegroup, the dimer is likely a crystallographic artefact, where it looks like the monomer is rotated by ~ 180 degrees around the Y axis.
I am assessing ligand binding and each of the monomers display density at the site but it is not as clear as I would like. A polder map does help things but I was wondering if it is feasible, or if there are any tools, that can be used to improve density based of the fact there are two molecles present. Is there some way to sum (probably the wrong word here) the densities at the binding site?
Kind regards,
Kyle
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