Tell the editor to find a new reviewer. And cite Kay's paper, the one Graeme linked to.
On 28/02/2020 08:22, dusan turk wrote: > Hi, > > Browsing through the recent discussion on EM data resolution cutoff it > occurred to me that the X-ray diffraction community isn’t that unanimous > either. > > My stand: > > When the default resolution cutoff provided with the data processing software > in electron density map calculation and refinement delivers quality maps > noisier than expected and/or too high R-factors I start adjusting the > resolution cutoff by lowering the resolution and trying alternative space > group. Hence, I allow the data processing programs to suggest where to draw > the line (be it CC1/2, I/sigI, R merge, R sym, R p.i.m. and R r.i.m, …) , > unless there are problems. > > Doing so, I came into a dispute with a referee who shaped his request: > > "It is well accepted that the criteria for resolution cutoff should consider > both I/SigI and Rmerge for the outer most shell. For data sets collected at > synchrotron sources, the criteria of I/SigI > 5 and Rmerge <50% can be taken > as a good practical reference.” > > So where do we stand? Which are the most objective criteria for resolution > cutoff to be used in diffraction data processing? Which number of shells to > use when calculating the statistics? Do we have a consensus? > > best wishes, > > dusan turk > > > > Dr. Dusan Turk, Prof. > Head of Structural Biology Group http://stef.ijs.si/ > Head of Centre for Protein and Structure Production > Centre of excellence for Integrated Approaches in Chemistry and Biology of > Proteins, Scientific Director > http://www.cipkebip.org/ > e-mail: dusan.t...@ijs.si > phone: +386 1 477 3857 Dept. of Biochem.& Mol.& Struct. Biology > fax: +386 1 477 3984 Jozef Stefan Institute > Jamova 39, 1 000 Ljubljana,Slovenia > Skype: dusan.turk (voice over internet: www.skype.com > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
