Dear All,
I have a situation, where a mutant protein does not exhibit
any heat change upon titration with cognate ligand in the ITC experiment.
However, it co-crystallizes with the respective cognate ligand. Also, the
cocrystal structure reveals the conservation of the hydrogen bonding
networks except for the mutated residues. I would like to know the possible
reason for the no heat change in the ITC experiment.
Looking forward to hearing from you.
--
*-----*
*With Kind Regards*
*Monika Chandravanshi*
*PhD Scholar, *
*Department of Biosciences and Bioengineering*
*Indian Institute of Technology Guwahati, Guwahati India*
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