Here's a few examples: https://www.rcsb.org/structure/1A2K The crystal has an A3B2 stoichiometry. The paper describes how they find A2B2 in solution, concluding the extra A molecule comes from "fortuitous packing" in the crystal
https://www.rcsb.org/structure/2RBL A design protein, but with very similar crystal packing as described in the original post: a domain-swapped dimer and a non-swapped monomer. https://www.rcsb.org/structure/1A99 Both dimers and monomers in the crystal On Fri, 31 Jan 2020 at 09:46, Diana Tomchick < diana.tomch...@utsouthwestern.edu> wrote: > Here’s two examples of heterooligomers that crystallized in a lattice with > an extra monomer of one of the proteins. In both cases this was an > unexpected result, but easily explained due to the low micro molar > affinities for the complex. > > 4PKY > 2CJS > > Diana > > ************************************************** > Diana R. Tomchick > Professor > Departments of Biophysics and Biochemistry > UT Southwestern Medical Center > 5323 Harry Hines Blvd. > Rm. ND10.214A > Dallas, TX 75390-8816 > diana.tomch...@utsouthwestern.edu > (214) 645-6383 (phone) > (214) 645-6353 (fax) > > On Jan 31, 2020, at 9:23 AM, Kluenemann, Thomas < > thomas.kluenem...@helmholtz-hzi.de> wrote: > > > EXTERNAL MAIL > > Dear all, > > We recently solved a the structure of a small c-type cytochrome. We > observed, that of the eleven chains in the asymmetric unit ten form 3D > domain swapped dimers by exchanging an α-helix. The eleventh chain is > present as a monomer. Based on the anomalous iron signal and the chain > tracing we are sure that no chain was missed. > I tried to find other examples in the PDB, were one crystal is made of > different homo- or heterooligomers. I only found proteins with partial > occupied peptide binding sites, which is not what I am looking for. Does > anyone know of a case were the presence of different homo- or > heterooligomers is required to form the crystal? > > Best regards, > Thomas Klünemann > > > > ------------------------------ > > Helmholtz-Zentrum für Infektionsforschung GmbH | Inhoffenstraße 7 | 38124 > Braunschweig | www.helmholtz-hzi.de > > Vorsitzende des Aufsichtsrates: Frau MinDir'in Prof. Dr. Veronika von > Messling > Stellvertreter: MinDirig Rüdiger Eichel, Niedersächsisches Ministerium für > Wissenschaft und Kultur > Geschäftsführung: Prof. Dr. Dirk Heinz; Silke Tannapfel > Gesellschaft mit beschränkter Haftung (GmbH) > Sitz der Gesellschaft: Braunschweig > Handelsregister: Amtsgericht Braunschweig, HRB 477 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > CAUTION: This email originated from outside UTSW. Please be cautious of > links or attachments, and validate the sender's email address before > replying. > > > ------------------------------ > > UT Southwestern > > Medical Center > > The future of medicine, today. > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
