Here’s two examples of heterooligomers that crystallized in a lattice with an extra monomer of one of the proteins. In both cases this was an unexpected result, but easily explained due to the low micro molar affinities for the complex.
4PKY 2CJS Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry UT Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu<mailto:diana.tomch...@utsouthwestern.edu> (214) 645-6383 (phone) (214) 645-6353 (fax) On Jan 31, 2020, at 9:23 AM, Kluenemann, Thomas <thomas.kluenem...@helmholtz-hzi.de<mailto:thomas.kluenem...@helmholtz-hzi.de>> wrote: EXTERNAL MAIL Dear all, We recently solved a the structure of a small c-type cytochrome. We observed, that of the eleven chains in the asymmetric unit ten form 3D domain swapped dimers by exchanging an α-helix. The eleventh chain is present as a monomer. Based on the anomalous iron signal and the chain tracing we are sure that no chain was missed. I tried to find other examples in the PDB, were one crystal is made of different homo- or heterooligomers. I only found proteins with partial occupied peptide binding sites, which is not what I am looking for. Does anyone know of a case were the presence of different homo- or heterooligomers is required to form the crystal? Best regards, Thomas Klünemann ________________________________ Helmholtz-Zentrum für Infektionsforschung GmbH | Inhoffenstraße 7 | 38124 Braunschweig | www.helmholtz-hzi.de<http://www.helmholtz-hzi.de/> Vorsitzende des Aufsichtsrates: Frau MinDir'in Prof. Dr. Veronika von Messling Stellvertreter: MinDirig Rüdiger Eichel, Niedersächsisches Ministerium für Wissenschaft und Kultur Geschäftsführung: Prof. Dr. Dirk Heinz; Silke Tannapfel Gesellschaft mit beschränkter Haftung (GmbH) Sitz der Gesellschaft: Braunschweig Handelsregister: Amtsgericht Braunschweig, HRB 477 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 CAUTION: This email originated from outside UTSW. Please be cautious of links or attachments, and validate the sender's email address before replying. ________________________________ UT Southwestern Medical Center The future of medicine, today. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
