?The interface doesn't appear to be extensive. What is the buried surface area? Is the molecule a dimer in solution, or is the dimerization caused by crystal packing?
Reza Khayat, PhD Assistant Professor City College of New York Department of Chemistry New York, NY 10031 ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Ronald E. Stenkamp <stenk...@uw.edu> Sent: Friday, September 27, 2019 11:22 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [EXTERNAL] Re: [ccp4bb] Reg: water pentagon at dimer interface I don't know about the myth thing, but I remember Martha Teeter describing pentagons of waters in crambin. Here's a reference: Water Structure of a Hydrophobic Protein at Atomic Resolution: Pentagon Rings of WaterMolecules in Crystals of Crambin M. M. Teeter Proceedings of the National Academy of Sciences of the United States of America, 1984, 81(1), 6014-6018. Ron From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Vijaykumar Pillalamarri Sent: Friday, September 27, 2019 4:34 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Reg: water pentagon at dimer interface Dear Community, I solved the structure of a protein from vibrio. There are two molecules in the asymmetric unit of this protein. At the dimer interface, the C-termini of both the chains interact with each other with the help of five water molecules that form a pentagon. I have attached an image showing both the chains and stereo image of dimer interface in the inset. I was wondering if there is any significance to this or if there is any relevant literature that explains this behavior. Thank you Vijaykumar Pillalamarri C/O: Dr. Anthony Addlagatta Principal Scientist CSIR-IICT, Tarnaka Hyderabad, India-500007 Mobile: +918886922975 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMGaQ&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=jnChHHhlO3afLT8YM6exk1Sws6Gj2KvncF-wTMonStw&s=1N2BDqrk_l4Qzyf-cBJpruxzQf_Oa3ykGbM88vou_Yc&e=> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMGaQ&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=jnChHHhlO3afLT8YM6exk1Sws6Gj2KvncF-wTMonStw&s=1N2BDqrk_l4Qzyf-cBJpruxzQf_Oa3ykGbM88vou_Yc&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
