I can share what has worked for my crystals : 1. You can put a grid across your condition with same or altered drop ratios.
2. You can try micro seeding. (This has given me the best results so far). 3. You can try Hampton's additive screen. On Sun, Sep 8, 2019 at 10:09 AM Prem Prakash <prem...@gmail.com> wrote: > Dear all, > Sorry for a trivial query. I am trying to Co-crystallize my protein with > its substrate (peptide) using commercial screenings. In one condition of > JCSG plus (Molecular Dimension) that contains 0.2 M Magnesium chloride > hexahydrate, 0.1 M Tris 8.5 50 % v/v Ethylene glycol, I got needle like > crystals (picture attached). Does anyone have idea to optimize such needles > into better crystals. I would appreciate all your suggestions. > > Thank you > With kind regards, > Prem Prakash (Ph.D.) > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- Regards Chitra ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
