You could always make a 96 or a 24 well narrow spectrum screen around this condition, and then check if the morphology changes or not. What I have seen is that they work better if you tweak your drop ratios too. After such procedures, you could try using Hamptons Additive screen on top of your optimized condition.
Get Outlook for Android<https://aka.ms/ghei36> ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Prem Prakash <prem...@gmail.com> Sent: Sunday, September 8, 2019 10:09:29 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Optimization from needle shaped crystals Dear all, Sorry for a trivial query. I am trying to Co-crystallize my protein with its substrate (peptide) using commercial screenings. In one condition of JCSG plus (Molecular Dimension) that contains 0.2 M Magnesium chloride hexahydrate, 0.1 M Tris 8.5 50 % v/v Ethylene glycol, I got needle like crystals (picture attached). Does anyone have idea to optimize such needles into better crystals. I would appreciate all your suggestions. Thank you With kind regards, Prem Prakash (Ph.D.) ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
