Dear all, I am trying to solve the phases of the following SeMet data, but so far unsuccessfully. Suggestions are very welcome. Please see below some details about the case.
The statistics below is from a merged data from different kappas of the same crystal to increase redundancy. We used the fixed energy 12675 eV since the fluorescence detector was not working at the used beamline to get best energy values for this crystal. Xtriage did not indicate any crystallographic pathology, except moderate anisotropy. The unit cells parameters are 118.72 151.82 167.05 90.000 90.000 90.000 (P212121) containing from 8 to 12 molecules in the asymmetric unit. The protein has ~28.5 kDa and 10 Met residues, excluding those from the N- and C-termini, probably with low occupancy. Thus, something 80 to 120 scatterers are expected. Phenix_anomalous_signal indicates a probability of 99% to solve it and the anomalous signal is theoretically in a very good range. I have tried SHELXD with different resolutions and number of sites. I have also used Hyss. But all attempts failed. Thanks in advance Mario [cid:image001.png@01D53D5A.1C2140F0] Aviso Legal: Esta mensagem e seus anexos podem conter informa??es confidenciais e/ou de uso restrito. Observe atentamente seu conte?do e considere eventual consulta ao remetente antes de copi?-la, divulg?-la ou distribu?-la. Se voc? recebeu esta mensagem por engano, por favor avise o remetente e apague-a imediatamente. Disclaimer: This email and its attachments may contain confidential and/or privileged information. Observe its content carefully and consider possible querying to the sender before copying, disclosing or distributing it. If you have received this email by mistake, please notify the sender and delete it immediately. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
