Any anomalous diffraction? On Tue, 9 Jul 2019 at 10:32, Lumbini Yadav <lumbin...@gmail.com> wrote:
> Dear all, > > > > We have found a huge Fo-Fc density close to cysteine residue (see attached > image) in the structure with resolution of 1.2A. In the crystallization > condition, we have PEG 3350, Potassium phosphate monobasic, glycerol and > protein was in Tris and NaCl. Before freezing the crystals were soaked in > mother liquor containing sodium dithionite. > > > > I have tried different modified cysteine (CSX, CSO, OCS, CME, CSS, SNC, CSD, > CXM, SCH, CSU) from the library and also SO3, SO2 and peroxide. But in > all the screenings we do see some part of Fo-Fc density unaddressed at 3 > sigma. > > > > Does anyone have an idea about what this density could be? Covalent > modification? > > > > Thanks. > > > > Kind regards, > > Lumbini > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
