And so does the CCP4I2 GUI - ... Eleanor On Mon, 18 Mar 2019 at 08:39, Eugene Osipov <e.m.osi...@gmail.com> wrote:
> Dear Aleix, > what is in the buffer and crystallization conditions? I have seen > anomalous signal at 0.99 angstroms because my crystallization conditions > contain NaI. And F'' (I) is around 3 at this wavelength. > I would follow Eleanor advice. > OR > Try to generate anomalous map if you can solve your structure by molecular > replacement. I do not know about CCP4, but phenix.refine generate anomalous > map coeff-s if you refine against anomalous data, i.e. I(+)/I(-) > > Best wishes, > > пн, 18 мар. 2019 г. в 09:22, Eleanor Dodson < > 0000176a9d5ebad7-dmarc-requ...@jiscmail.ac.uk>: > >> Can you find the position of the anom scatterer used SHELXC/D/E or some >> other tool.There is no need to know the element name to use it for phasing! >> Eleanor >> >> PS However the existence of an "anom signal" just meand that I+ differs >> systematically from I- and there can be other experimental reasons for that >> appearing.. >> >> On Fri, 15 Mar 2019 at 22:08, Nukri Sanishvili <sannu...@gmail.com> >> wrote: >> >>> Hi Aleix, >>> Can you describe how you did the fluorescence scan, or share the plot >>> itself? I am asking this because you say you could discard Fe, Zn and As/Se >>> but you don't say anything about Cu which is in between these elements. >>> If you tried but could not discard Cu, then it could be your "Culprit". >>> Best, >>> Nukri >>> >>> On Fri, Mar 15, 2019 at 4:17 PM Aleix Tarrés Solé <atarre...@gmail.com> >>> wrote: >>> >>>> Dear colleagues: >>>> Hereby, I would like to expose a problem I encountered during the data >>>> processing of one of my crystals. Recently I collected a full dataset of a >>>> native protein-DNA crystal at an energy of 12667KeV. To my surprise, after >>>> processing with XDS I can see that I have significant values of anomalous >>>> correlation up to 5 Angstrom resolution. Using the fluorescence scan on the >>>> beam, I could discard the presence of the following heavy atoms that could >>>> have ended up in my drop due to cross-contamination; Se, As, Fe, Zn and Pr. >>>> So far, my attempts to use this dataset for phasing have been sadly >>>> unsuccessful. I would like to know if somebody has experience in this kind >>>> of problem and can gently help with some advices. >>>> >>>> Thank you all in advance >>>> >>>> Aleix >>>> >>>> >>>> ------------------------------ >>>> >>>> To unsubscribe from the CCP4BB list, click the following link: >>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >>>> >>> >>> ------------------------------ >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >>> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > > > -- > Evgenii Osipov > Laboratory for Biocrystallography, > Department of Pharmaceutical Sciences, > KU Leuven O&N2 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
