Can you find the position of the anom scatterer used SHELXC/D/E or some other tool.There is no need to know the element name to use it for phasing! Eleanor
PS However the existence of an "anom signal" just meand that I+ differs systematically from I- and there can be other experimental reasons for that appearing.. On Fri, 15 Mar 2019 at 22:08, Nukri Sanishvili <sannu...@gmail.com> wrote: > Hi Aleix, > Can you describe how you did the fluorescence scan, or share the plot > itself? I am asking this because you say you could discard Fe, Zn and As/Se > but you don't say anything about Cu which is in between these elements. > If you tried but could not discard Cu, then it could be your "Culprit". > Best, > Nukri > > On Fri, Mar 15, 2019 at 4:17 PM Aleix Tarrés Solé <atarre...@gmail.com> > wrote: > >> Dear colleagues: >> Hereby, I would like to expose a problem I encountered during the data >> processing of one of my crystals. Recently I collected a full dataset of a >> native protein-DNA crystal at an energy of 12667KeV. To my surprise, after >> processing with XDS I can see that I have significant values of anomalous >> correlation up to 5 Angstrom resolution. Using the fluorescence scan on the >> beam, I could discard the presence of the following heavy atoms that could >> have ended up in my drop due to cross-contamination; Se, As, Fe, Zn and Pr. >> So far, my attempts to use this dataset for phasing have been sadly >> unsuccessful. I would like to know if somebody has experience in this kind >> of problem and can gently help with some advices. >> >> Thank you all in advance >> >> Aleix >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
