Hello Reza,

Have you tried ConSurf?
http://consurf.tau.ac.il/2016/

I just noticed they now also have precalculated results for proteins deposited 
in the PDB:
http://bental.tau.ac.il/new_ConSurfDB/

Hope this helps,

Guillaume


> On Mar 16, 2019, at 17:42, Reza Khayat <rkha...@ccny.cuny.edu> wrote:
> 
> ​Hi,
> 
> 
> 
> Can someone direct me to a program that can replace the B-factors of a PDB 
> with sequence variability calculated from a sequence alignment? I'm have 
> tried the PVS server, but my sequence alignment is too large for its liking. 
> Thanks.
> 
> 
> 
> Best wishes,
> Reza
> 
> 
> 
> Reza Khayat, PhD
> Assistant Professor 
> City College of New York
> Department of Chemistry
> New York, NY 10031
> 
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