Dear everybody who reacted, It seems that when the His6 construct crystallizes, there is a fair chance that the his10 construct will crystallize as well. However, as usual in crystallography there is no guarantee, so I have added a TEV site to the construct in order to be able to remove the tag if necessary.
Thank you all very much! Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Savvas Savvides Gesendet: Dienstag, 19. September 2017 16:12 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] His-6 versus His-10 tag Hi Herman the survey article by Carson in Acta D DOI: 10.1107/S0907444906052024<https://urldefense.proofpoint.com/v2/url?u=https-3A__doi.org_10.1107_S0907444906052024&d=DwMFAg&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=f0yyUbNahC99mMQ7mNgibBIjHQnTAYPdgDT1unpORi8&s=0ue_qfHHBCOr_J006W2KUHZYoPsjHWboidAAqLjps8g&e=> from a decade ago may provide relevant information/insights. best wishes Savvas On 19 Sep 2017, at 12:10, herman.schreu...@sanofi.com<mailto:herman.schreu...@sanofi.com> wrote: Dear BB, We are planning the production of a protein for crystallization. From literature, we know that the construct with a 6-histidine tag crystallizes. However, for other biophysical measurements, we would prefer to have a 10-histidine tag. Does anyone has experience with His-6 versus His-10 tags in terms of crystallization success? Thanks for your help! Herman
