Dear Narayanan Which kind of resin are you using ? In my lab we work with proteins that bind dextran which is often used as crosslinker in chromatography resins. Crosslinked resins have a highly reticulated structure made of sugars that can be quite similar to peptidoglycan... As a test, you could try to inject your protein in the presence of glucose (or another sugar) to compete with this interaction. Changing pH and NaCl concentration might help too.
Good luck, Gianluca. Il 15/Set/2017 01:54 PM, "Narayanan Ramasubbu" <ramas...@sdm.rutgers.edu> ha scritto: > Hi. We are working on a periplasmic protein that breaks naked glycans in > peptidoglycans. There is truncated structure available but our target is > the full length protein. The difficulty us that it strongly binds to the > resin with or without his.tag. Changing the resin to acrylamide did not > help. > Has anyone come across similar problem and how was it resolved. > The pdb structure is the catalytic domain and mussing a region that, in my > opinion, binds to the resin. > Thank you in advance > Sent from my iPhone
