change buffer，or use different resin matrix from another compny
发自网易邮箱大师 在2017年09月15日 19:53，Narayanan Ramasubbu 写道： Hi. We are working on a periplasmic protein that breaks naked glycans in peptidoglycans. There is truncated structure available but our target is the full length protein. The difficulty us that it strongly binds to the resin with or without his.tag. Changing the resin to acrylamide did not help. Has anyone come across similar problem and how was it resolved. The pdb structure is the catalytic domain and mussing a region that, in my opinion, binds to the resin. Thank you in advance Sent from my iPhone
