Hi, This might indicate that the binding affinity of your complex is not high enough for it to resist the gel filtration dilution. Maybe running a native PAGE gel would allow you to visualise a complex.
Best A > On 18 Jul 2017, at 10:25, 高艺娜 <gaoy...@cau.edu.cn> wrote: > > Hi all , > > It has been reported the Negative stain EM of a protein A-B complex, but > according to my gel filtration results (I purified A and B respectively for > incubation) , I found that A could not bind to B, of course I tried different > buffer condition with various pH value, even the binding condition only had > 50 mm Kcl. Do you have any suggestion or methods that I can try to get the > protein A-B complex? > > Any suggestion is welcome， > > Thank you all , > > Best, ________________________________ CONFIDENTIALITY: This email is intended solely for the person(s) named and may be confidential and/or privileged. If you are not the intended recipient, please delete it, notify us and do not copy, use, or disclose its contents. Towards a sustainable earth: Print only when necessary. Thank you.
