Hi all , It has been reported the Negative stain EM of a protein A-B complex, but according to my gel filtration results (I purified A and B respectively for incubation) , I found that A could not bind to B, of course I tried different buffer condition with various pH value, even the binding condition only had 50 mm Kcl. Do you have any suggestion or methods that I can try to get the protein A-B complex?
Any suggestion is welcome， Thank you all , Best,
