Dear All,
I have a very elementary question. At what stage of refinement is it appropriate to start adding solvent (water). For example, during refinement of a structure, I was able to build ~ 90% of residues. The density of the remaining residues is not complete. If I take a guess and add residues based on my knowledge of the sequence, the r-free increases. Since I see no further way to improve the maps (any suggestions?), should I start adding water in the hope of improving the overall density and revealing the missing bits. I would really appreciate any inputs. thank you Abhishek
