Dear Praveen, We have very good experience with protein ligand interaction with Biacore 3000 using CM5 chip. The NTA chip is prone to nonspecific interactions and give erroneous results. However the gold standard is ITC since it is more sensitive than Biacore 3000. One quick note for using Biacore 3000 is to optimize the buffer. HBS appeared nice for us. Hope that helps.
Cheers!! Debasish CSIR- Senior Research Fellow (PhD Scholar) C/o: Dr. Akash Ranjan Computational and Functional Genomics Group Centre for DNA Fingerprinting and Diagnostics Hyderabad, INDIA Email(s): dkgh...@cdfd.org.in, dgho...@gmail.com Telephone: 0091-9088334375 (M), 0091-40-24749396 (Lab) Lab URL: http://www.cdfd.org.in/labpages/computational_functional_genomics.html ----- Original Message ----- From: Praveen Tripathi <tripathipraveen2...@gmail.com> To: CCP4BB@JISCMAIL.AC.UK Sent: Fri, 31 Mar 2017 10:31:43 +0530 (IST) Subject: [ccp4bb] Protein Ligand interaction using SPR technique Dear all, Sorry for off-topic question. I want to study protein interaction with few small molecules using SPR (Machine- *BIACORE 3000*). The recombinant protein expressed in bacterial expression system is of 92 kDa (with His tag), pI= 9. *Question-* 1. What should be the chip of choice- *NTA chip or CM5 chip* of GE healthcare? 2. Is there any alternative available for chips and machine? Thanks in advance. Regards ---------------------------- Praveen
