Hi Praveen, The BiaCore 3000 is an older model, and is not optimised for protein/small molecule interactions. The BiaCore T-100 and T-200 have enhanced sensitivity and are better suited small molecules (assuming you have to use SPR).
As for chip choice, it depends an awful lot on the behaviour of your protein. NTA imo is not great as you will always have some loss if protein during the experiment - covalent immobilisation to CM5 is preferable. Do you have access to ITC? You might consider this if protein is easy to make. It's a better experiment than SPR, but is much more thirsty. HTH, Dave On Fri, 31 Mar 2017, 06:04 Praveen Tripathi, <tripathipraveen2...@gmail.com> wrote: > Dear all, > Sorry for off-topic question. > I want to study protein interaction with few small molecules using SPR > (Machine- *BIACORE 3000*). > > The recombinant protein expressed in bacterial expression system is of 92 > kDa (with His tag), pI= 9. > > *Question-* > 1. What should be the chip of choice- *NTA chip or CM5 chip* of GE > healthcare? > 2. Is there any alternative available for chips and machine? > > Thanks in advance. > > Regards > ---------------------------- > Praveen > -- [image: --] David Briggs PhD [image: https://]about.me/david_briggs <https://about.me/david_briggs?promo=email_sig&utm_source=email_sig&utm_medium=email_sig&utm_campaign=external_links>
