Hi Sunanda, If you model is good, high average B-factors are just caused by the quality of your data. You can sometimes improve this a bit by careful reprocessing (e.g. by not using the last frames where radiation damage really kicked in). Sometimes you can have a better crystal and your data recording strategy can also help a bit. That said, sometimes you just have to work with what you got. If you just make the best model you can, it will be reliable enough to describe you protein, just not with very high precision on atomic distances and such. The fact that you N-terminus and two loops have higher B-factors than the rest is hardly shocking. These are the most flexible bits of your protein.
Cheers, Robbie Sent from my Windows 10 phone Van: sunanda williams<mailto:sunanda.willi...@gmail.com> Verzonden: donderdag 13 oktober 2016 05:10 Aan: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Onderwerp: [ccp4bb] High B factor Hi all, I have a structure at 3.0 A and R/Rfree of 24/28. The mean B value is around 98. The B value is especially high at the N terminus and two loop regions (around 120-150 AA). The rest of the structure averaged around 70-75. Has anyone faced such a scenario? How reliable is the structure and is there any way to better the B factors. Any help is appreciated. Thank you!!
